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human trim33  (Addgene inc)
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Addgene inc human trim33
<t>TRIM33</t> is phosphorylated by RSK at S1119. A , HEK293 cells were transfected with HA-tagged TRIM33, serum-starved overnight, and stimulated for 10 or 20 min with fetal bovine serum (FBS; 10%), EGF (25 ng/ml), insulin (100 nM), or PMA (50 ng/ml). Immunoprecipitated TRIM33 was then assayed for phosphorylation with a phospho-motif antibody that recognizes the RxxS∗/T∗ consensus motif. B , same as in ( A ), except cells were pretreated with PD184352 (10 μM) or BI-D1870 (10 μM) for 30 min prior to PMA stimulation. C , as in ( A ), except cells were transfected with HA-tagged TRIM33 WT or the S1119A unphosphorylable mutant. D , detection of phospho-RxxS∗/T∗ in TRIM33-WT and -S1119A in WT and RSK-DKO HeLa cells. Cells transfected with the HA-tagged TRIM33 constructs were stimulated for 30 min with PMA prior to HA immunoprecipitation. PMA, phorbol 12-myristate 13-acetate; RSK, ribosomal S6 kinase.
Human Trim33, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
human trim33 - by Bioz Stars, 2026-07
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TRIM33 is phosphorylated by RSK at S1119. A , HEK293 cells were transfected with HA-tagged TRIM33, serum-starved overnight, and stimulated for 10 or 20 min with fetal bovine serum (FBS; 10%), EGF (25 ng/ml), insulin (100 nM), or PMA (50 ng/ml). Immunoprecipitated TRIM33 was then assayed for phosphorylation with a phospho-motif antibody that recognizes the RxxS∗/T∗ consensus motif. B , same as in ( A ), except cells were pretreated with PD184352 (10 μM) or BI-D1870 (10 μM) for 30 min prior to PMA stimulation. C , as in ( A ), except cells were transfected with HA-tagged TRIM33 WT or the S1119A unphosphorylable mutant. D , detection of phospho-RxxS∗/T∗ in TRIM33-WT and -S1119A in WT and RSK-DKO HeLa cells. Cells transfected with the HA-tagged TRIM33 constructs were stimulated for 30 min with PMA prior to HA immunoprecipitation. PMA, phorbol 12-myristate 13-acetate; RSK, ribosomal S6 kinase.

Journal: The Journal of Biological Chemistry

Article Title: Identification of RSK substrates using an analog-sensitive kinase approach

doi: 10.1016/j.jbc.2024.105739

Figure Lengend Snippet: TRIM33 is phosphorylated by RSK at S1119. A , HEK293 cells were transfected with HA-tagged TRIM33, serum-starved overnight, and stimulated for 10 or 20 min with fetal bovine serum (FBS; 10%), EGF (25 ng/ml), insulin (100 nM), or PMA (50 ng/ml). Immunoprecipitated TRIM33 was then assayed for phosphorylation with a phospho-motif antibody that recognizes the RxxS∗/T∗ consensus motif. B , same as in ( A ), except cells were pretreated with PD184352 (10 μM) or BI-D1870 (10 μM) for 30 min prior to PMA stimulation. C , as in ( A ), except cells were transfected with HA-tagged TRIM33 WT or the S1119A unphosphorylable mutant. D , detection of phospho-RxxS∗/T∗ in TRIM33-WT and -S1119A in WT and RSK-DKO HeLa cells. Cells transfected with the HA-tagged TRIM33 constructs were stimulated for 30 min with PMA prior to HA immunoprecipitation. PMA, phorbol 12-myristate 13-acetate; RSK, ribosomal S6 kinase.

Article Snippet: The original plasmid encoding human TRIM33 (pCS2-FLAG-hEcto/Tif1g) was obtained from Dr Stefano Piccolo through Addgene (#20902).

Techniques: Transfection, Immunoprecipitation, Phospho-proteomics, Mutagenesis, Construct